HTqPCR

Automated analysis of high-throughput qPCR data

Analysis of Ct values from quantitative real-time PCR (qPCR) assays. Data can be analyzed in high throughput across multiple conditions or replicates, and in spatially-defined formats such ABI TaqMan Low Density Arrays and conventional 96- or 384-well plates. HTqPCR handles data loading, quality assessment, normalization, visualization and parametric or non-parametric testing for statistical significance in Ct values between features (e.g. genes, microRNAs).

Author	Heidi Dvinge, Paul Bertone
Maintainer	Heidi Dvinge

To install this package, start R and enter:

    source("http://bioconductor.org/biocLite.R")
    biocLite("HTqPCR")

Documentation

qPCR analysis in R	PDF	R Script
Reference Manual

Details

biocViews	MicrotitrePlateAssay , DifferentialExpression , GeneExpression , DataImport , QualityControl , Preprocessing , Bioinformatics , Visualization , MultipleComparisons
Depends	Biobase , RColorBrewer , limma
Imports	affy , Biobase , gplots , graphics , grDevices , limma , methods , RColorBrewer , stats , utils
Suggests	statmod
System Requirements
License	Artistic-2.0
URL	http://www.ebi.ac.uk/bertone/software
Depends On Me
Imports Me
Suggests Me

Package Downloads

Package source	HTqPCR_1.2.0.tar.gz
Windows 32-bit binary	HTqPCR_1.2.0.zip
Windows 64-bit binary	HTqPCR_1.2.0.zip
MacOS X 10.5 (Leopard) binary	HTqPCR_1.2.0.tgz
Package Downloads Report	Downloads Stats