Here, we describe the workflow to run variance-sensitive clustering on data from a quantitative omics experiments. In principle, this can be any multi-dimensional data set containing quantitative and optionally replicated values. This vignette is distributed under a CC BY-SA license.

vsclust 1.7.0

Clustering is a method to identify common pattern in highly dimensional data. This can be for example genes or proteins with similar quantitative changes, thus providing insights into the affected biological pathways.

Despite of numerous clustering algorithms, they do not account for feature variance, i.e. the uncertainty in the measurements across the different experimental conditions. VSClust determines the characteristic patterns in high-dimensional data while accounting for feature variance that is given through replicated measurements.

Here, we present an example script to run the full clustering analysis using
the `vsclust`

library. The same can be done by running the Shiny app (e.g. via
its docker image or on ), or the
corresponding command line script. For the source code, see
.

Use the common Bioconductor commands for installation:

```
if (!require("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install("vsclust")
```

The full functionality of this vignette can be obtained by additionally
installing and loading the packages `matrixStats`

and `clusterProfiler`

Here, we define the different parameters for the example data set
`protein_expressions`

. In the command-line version of VSClust (“runVSClust.R”),
they can be given via yaml file.

**Comments:**

A. Data sets with different numbers of replicates per condition need to be adapted to contain the same number of columns per condition. These can be done by either removing excess replicates or adding empty columns.

B. We assume the input data to be of the following format: A1, B1, C1, …, A2, B2, C2, …, where letters denote sample type and numbers are the different replicates.

C. If you prefer to estimate feature variance different, use averages and add
an estimate for the standard deviation as last column. You will need to set the
last option of `PreparedForVSClust`

to `FALSE`

.

D. If you don’t have replicates, use the same format as in C. and set the standard deviations to 1.

```
#### Input parameters, only read when now parameter file was provided
## All principal parameters for running VSClust can be defined as in the
## shinyapp at computproteomics.bmb.sdu.dk/Apps/VSClust
# name of study
Experiment <- "ProtExample"
# Number of replicates/sample per different experimental condition (sample
# type)
NumReps <- 3
# Number of different experimental conditions (e.g. time points or sample
# types)
NumCond <- 4
# Paired or unpaired statistical tests when carrying out LIMMA for
# statistical testing
isPaired <- FALSE
# Number of threads to accelerate the calculation (use 1 in doubt)
cores <- 1
# If 0 (default), then automatically estimate the cluster number for the
# vsclust
# run from the Minimum Centroid Distance
PreSetNumClustVSClust <- 0
# If 0 (default), then automatically estimate the cluster number for the
# original fuzzy c-means from the Minimum Centroid Distance
PreSetNumClustStand <- 0
# max. number of clusters when estimating the number of clusters. Higher
# numbers can drastically extend the computation time.
maxClust <- 10
```

At first, we load the example proteomics data set and carry out statistical
testing of all conditions version the first based on the LIMMA moderated t-test.
The data consists of mice fed with four different diets (high fat, TTA, fish oil
and TTA\(+\)fish oil).
Understand more about the data set with `?protein_expressions`

This will calculate the false discovery rates for the differentially regulated features (pairwise comparisons versus the first “high fat” condition) and most importantly, their expected individual variances, to be used in the variance-sensitive clustering. These variances can also be uploaded separately via a last column containing them as individual standard deviations.

The `PrepareForVSClust`

function also creates a PCA plot to assess variability
and control whether the samples have been loaded correctly (replicated samples
should form groups).

After estimating the standard deviations, the matrix consists of the averaged quantitative feature values and a last column for the standard deviations of the features.

```
data(protein_expressions)
dat <- protein_expressions
#### running statistical analysis and estimation of individual variances
statOut <- PrepareForVSClust(dat, NumReps, NumCond, isPaired, TRUE)
```