if (!requireNamespace("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install("SingleCellMultiModal")library(MultiAssayExperiment)
library(SingleCellMultiModal)CITE-seq data are a combination of two data types extracted at the same time from the same cell. First data type is scRNA-seq data, while the second one consists of about a hundread of antibody-derived tags (ADT). In particular this dataset is provided by Stoeckius et al. (2017).
The user can see the available dataset by using the default options
CITEseq(DataType="cord_blood", modes="*", dry.run=TRUE, version="1.0.0")## Dataset: cord_blood## snapshotDate(): 2022-10-24## ah_id mode file_size rdataclass rdatadateadded rdatadateremoved
## 1 EH3795 scADT_Counts 0.2 Mb matrix 2020-09-23 <NA>
## 2 EH3796 scRNAseq_Counts 22.2 Mb matrix 2020-09-23 <NA>Or simply by setting dry.run = FALSE it downloads the data and creates the
MultiAssayExperiment object.
In this example, we will use one of the two available datasets scADT_Counts:
mae <- CITEseq(
DataType="cord_blood", modes="*", dry.run=FALSE, version="1.0.0"
)
mae## A MultiAssayExperiment object of 2 listed
## experiments with user-defined names and respective classes.
## Containing an ExperimentList class object of length 2:
## [1] scADT: matrix with 13 rows and 8617 columns
## [2] scRNAseq: matrix with 36280 rows and 8617 columns
## Functionality:
## experiments() - obtain the ExperimentList instance
## colData() - the primary/phenotype DataFrame
## sampleMap() - the sample coordination DataFrame
## `$`, `[`, `[[` - extract colData columns, subset, or experiment
## *Format() - convert into a long or wide DataFrame
## assays() - convert ExperimentList to a SimpleList of matrices
## exportClass() - save data to flat filesExample with actual data:
experiments(mae)## ExperimentList class object of length 2:
## [1] scADT: matrix with 13 rows and 8617 columns
## [2] scRNAseq: matrix with 36280 rows and 8617 columnsCheck row annotations:
rownames(mae)## CharacterList of length 2
## [["scADT"]] CD3 CD4 CD8 CD45RA CD56 CD16 CD10 CD11c CD14 CD19 CD34 CCR5 CCR7
## [["scRNAseq"]] ERCC_ERCC-00104 HUMAN_A1BG ... MOUSE_n-R5s25 MOUSE_n-R5s31Take a peek at the sampleMap:
sampleMap(mae)## DataFrame with 17234 rows and 3 columns
## assay primary colname
## <factor> <character> <character>
## 1 scADT CTGTTTACACCGCTAG CTGTTTACACCGCTAG
## 2 scADT CTCTACGGTGTGGCTC CTCTACGGTGTGGCTC
## 3 scADT AGCAGCCAGGCTCATT AGCAGCCAGGCTCATT
## 4 scADT GAATAAGAGATCCCAT GAATAAGAGATCCCAT
## 5 scADT GTGCATAGTCATGCAT GTGCATAGTCATGCAT
## ... ... ... ...
## 17230 scRNAseq TTGCCGTGTAGATTAG TTGCCGTGTAGATTAG
## 17231 scRNAseq GGCGTGTAGTGTACTC GGCGTGTAGTGTACTC
## 17232 scRNAseq CGTATGCCGTCTTCTG CGTATGCCGTCTTCTG
## 17233 scRNAseq TACACGACGCTCTTCC TACACGACGCTCTTCC
## 17234 scRNAseq ACACGACGCTCTTCCG ACACGACGCTCTTCCGThe scRNA-seq data are accessible with the name scRNAseq, which returns a
matrix object.
head(experiments(mae)$scRNAseq)[, 1:4]## CTGTTTACACCGCTAG CTCTACGGTGTGGCTC AGCAGCCAGGCTCATT
## ERCC_ERCC-00104 0 0 0
## HUMAN_A1BG 0 0 0
## HUMAN_A1BG-AS1 0 0 0
## HUMAN_A1CF 0 0 0
## HUMAN_A2M 0 0 0
## HUMAN_A2M-AS1 0 0 0
## GAATAAGAGATCCCAT
## ERCC_ERCC-00104 0
## HUMAN_A1BG 0
## HUMAN_A1BG-AS1 0
## HUMAN_A1CF 0
## HUMAN_A2M 0
## HUMAN_A2M-AS1 0The scADT data are accessible with the name scADT, which returns a
matrix object.
head(experiments(mae)$scADT)[, 1:4]## CTGTTTACACCGCTAG CTCTACGGTGTGGCTC AGCAGCCAGGCTCATT GAATAAGAGATCCCAT
## CD3 60 52 89 55
## CD4 72 49 112 66
## CD8 76 59 61 56
## CD45RA 575 3943 682 378
## CD56 64 68 87 58
## CD16 161 107 117 82Because of already large use of some methodologies (such as
in the SingleCellExperiment vignette or CiteFuse Vignette where the
SingleCellExperiment object is used for CITE-seq data,
we provide a function for the conversion of our CITE-seq MultiAssayExperiment
object into a SingleCellExperiment object with scRNA-seq data as counts and
scADT data as altExps.
sce <- CITEseq(DataType="cord_blood", modes="*", dry.run=FALSE, version="1.0.0",
DataClass="SingleCellExperiment")
sce## class: SingleCellExperiment
## dim: 36280 8617
## metadata(0):
## assays(1): counts
## rownames(36280): ERCC_ERCC-00104 HUMAN_A1BG ... MOUSE_n-R5s25
## MOUSE_n-R5s31
## rowData names(0):
## colnames(8617): CTGTTTACACCGCTAG CTCTACGGTGTGGCTC ... TACACGACGCTCTTCC
## ACACGACGCTCTTCCG
## colData names(0):
## reducedDimNames(0):
## mainExpName: NULL
## altExpNames(1): scADTsessionInfo()## R Under development (unstable) (2022-10-25 r83175)
## Platform: x86_64-pc-linux-gnu (64-bit)
## Running under: Ubuntu 22.04.1 LTS
##
## Matrix products: default
## BLAS: /home/biocbuild/bbs-3.17-bioc/R/lib/libRblas.so
## LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.10.0
##
## locale:
## [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
## [3] LC_TIME=en_GB LC_COLLATE=C
## [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
## [7] LC_PAPER=en_US.UTF-8 LC_NAME=C
## [9] LC_ADDRESS=C LC_TELEPHONE=C
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
##
## attached base packages:
## [1] stats4 stats graphics grDevices utils datasets methods
## [8] base
##
## other attached packages:
## [1] SingleCellMultiModal_1.11.0 MultiAssayExperiment_1.25.1
## [3] SummarizedExperiment_1.29.0 Biobase_2.59.0
## [5] GenomicRanges_1.51.0 GenomeInfoDb_1.35.0
## [7] IRanges_2.33.0 S4Vectors_0.37.0
## [9] BiocGenerics_0.45.0 MatrixGenerics_1.11.0
## [11] matrixStats_0.62.0 BiocStyle_2.27.0
##
## loaded via a namespace (and not attached):
## [1] DBI_1.1.3 bitops_1.0-7
## [3] formatR_1.12 rlang_1.0.6
## [5] magrittr_2.0.3 compiler_4.3.0
## [7] RSQLite_2.2.18 DelayedMatrixStats_1.21.0
## [9] png_0.1-7 vctrs_0.5.0
## [11] stringr_1.4.1 pkgconfig_2.0.3
## [13] SpatialExperiment_1.9.0 crayon_1.5.2
## [15] fastmap_1.1.0 magick_2.7.3
## [17] dbplyr_2.2.1 XVector_0.39.0
## [19] ellipsis_0.3.2 scuttle_1.9.0
## [21] utf8_1.2.2 promises_1.2.0.1
## [23] rmarkdown_2.17 purrr_0.3.5
## [25] bit_4.0.4 xfun_0.34
## [27] zlibbioc_1.45.0 cachem_1.0.6
## [29] beachmat_2.15.0 jsonlite_1.8.3
## [31] blob_1.2.3 later_1.3.0
## [33] rhdf5filters_1.11.0 DelayedArray_0.25.0
## [35] Rhdf5lib_1.21.0 BiocParallel_1.33.0
## [37] interactiveDisplayBase_1.37.0 parallel_4.3.0
## [39] R6_2.5.1 bslib_0.4.0
## [41] stringi_1.7.8 limma_3.55.0
## [43] jquerylib_0.1.4 Rcpp_1.0.9
## [45] bookdown_0.29 assertthat_0.2.1
## [47] knitr_1.40 R.utils_2.12.1
## [49] httpuv_1.6.6 Matrix_1.5-1
## [51] tidyselect_1.2.0 yaml_2.3.6
## [53] codetools_0.2-18 curl_4.3.3
## [55] lattice_0.20-45 tibble_3.1.8
## [57] withr_2.5.0 shiny_1.7.3
## [59] KEGGREST_1.39.0 evaluate_0.17
## [61] BiocFileCache_2.7.0 ExperimentHub_2.7.0
## [63] Biostrings_2.67.0 pillar_1.8.1
## [65] BiocManager_1.30.19 filelock_1.0.2
## [67] generics_0.1.3 RCurl_1.98-1.9
## [69] BiocVersion_3.17.0 sparseMatrixStats_1.11.0
## [71] xtable_1.8-4 glue_1.6.2
## [73] tools_4.3.0 AnnotationHub_3.7.0
## [75] locfit_1.5-9.6 rhdf5_2.43.0
## [77] grid_4.3.0 DropletUtils_1.19.0
## [79] AnnotationDbi_1.61.0 edgeR_3.41.0
## [81] SingleCellExperiment_1.21.0 GenomeInfoDbData_1.2.9
## [83] HDF5Array_1.27.0 cli_3.4.1
## [85] rappdirs_0.3.3 fansi_1.0.3
## [87] dplyr_1.0.10 R.methodsS3_1.8.2
## [89] sass_0.4.2 digest_0.6.30
## [91] dqrng_0.3.0 rjson_0.2.21
## [93] memoise_2.0.1 htmltools_0.5.3
## [95] R.oo_1.25.0 lifecycle_1.0.3
## [97] httr_1.4.4 mime_0.12
## [99] bit64_4.0.5Stoeckius, Marlon, Christoph Hafemeister, William Stephenson, Brian Houck-Loomis, Pratip K Chattopadhyay, Harold Swerdlow, Rahul Satija, and Peter Smibert. 2017. “Simultaneous Epitope and Transcriptome Measurement in Single Cells.” Nature Methods 14 (9): 865.