Bioconductor Developer Survey 2026 Now Open!

stPipe

This is the released version of stPipe; for the devel version, see stPipe.

All versions 3.24 (devel), 3.23 (release), 3.22

Upstream pre-processing for Sequencing-Based Spatial Transcriptomics


Bioconductor version: Release (3.23)

This package serves as an upstream pipeline for pre-processing sequencing-based spatial transcriptomics data. Functions includes FASTQ trimming, BAM file reformatting, index building, spatial barcode detection, demultiplexing, gene count matrix generation with UMI deduplication, QC, and revelant visualization. Config is an essential input for most of the functions which aims to improve reproducibility.

Author: Yang Xu [aut, cre] ORCID iD ORCID: 0009-0008-3274-6516 , Callum Sargeant [aut], Shian Su [aut], Luyi Tian [aut], Yunshun Chen [ctb], Matthew Ritchie [ctb, fnd]

Maintainer: Yang Xu <xu.ya at wehi.edu.au>

Citation (from within R, enter citation("stPipe")):

Yang Xu, Callum Sargeant, Shian Su, Luyi Tian. stPipe: Upstream pre-processing for Sequencing-Based Spatial Transcriptomics. doi:10.18129/B9.bioc.stPipe, R package version 1.2.0, https://bioconductor.org/packages/stPipe.

Generated from the package metadata; it may differ from the package's own citation.

Installation

To install this package, start R (version "4.6") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("stPipe")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("stPipe")
stPipe: A flexible and streamlined pipeline for processing sequencing-based spatial transcriptomics data HTML R Script
Reference ManualPDF
NEWSText

Details

biocViews Clustering, DataImport, GeneExpression, GenomeAnnotation, ImmunoOncology, Preprocessing, QualityControl, RNASeq, SequenceMatching, Sequencing, SingleCell, Software, Spatial, Transcriptomics, Visualization
Version1.2.0
In Bioconductor sinceBioC 3.22 (R-4.5) (1 year)
License GPL-3
Depends R (>= 4.5.0)
Imports basilisk, data.table, DropletUtils, dplyr, ggplot2, methods, pbmcapply, reticulate, rmarkdown, Rcpp, Rhtslib, Rsubread, Rtsne, Seurat, SeuratObject, scPipe, shiny, SummarizedExperiment, SingleCellExperiment, SpatialExperiment, stats, umap, yaml
System RequirementsGNU make
URLhttps://github.com/mritchielab/stPipe
Bug Reportshttps://github.com/mritchielab/stPipe/issues/new
See More
Suggests knitr, plotly, BiocStyle, testthat (>= 3.0.0)
Linking To Rcpp, Rhdf5lib, testthat, Rhtslib
Enhances
Depends On Me
Imports Me
Suggests Me
Links To Me
Build Report Build Report, r-universe

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package stPipe_1.2.0.tar.gz
Windows Binary (x86_64) stPipe_1.2.0.zip
macOS Binary (big-sur-x86_64) stPipe_1.2.0.tgz
macOS Binary (sonoma-arm64) stPipe_1.2.0.tgz
Source Repositorygit clone https://git.bioconductor.org/packages/stPipe
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/stPipe
Package Short Url https://bioconductor.org/packages/stPipe/
Package Downloads ReportDownload Stats