ddCt
The ddCt Algorithm for the Analysis of Quantitative Real-Time
PCR (qRT-PCR)
The Delta-Delta-Ct (ddCt) Algorithm is an approximation
method to determine relative gene expression with quantitative
real-time PCR (qRT-PCR) experiments. Compared to other
approaches, it requires no standard curve for each
primer-target pair, therefore reducing the working load and yet
returning accurate enough results as long as the assumptions of
the amplification efficiency hold. The ddCt package implements
a pipeline to collect, analyse and visualize qRT-PCR results,
for example those from TaqMan SDM software, mainly using the
ddCt method. The pipeline can be either invoked by a script in
command-line or through the API consisting of S4-Classes,
methods and functions.
| Author |
Rudolf Biczok, Markus Ruschhaupt and Jitao David Zhang |
| Maintainer |
Jitao David Zhang |
To install this package, start R and enter:
source("http://bioconductor.org/biocLite.R")
biocLite("ddCt")
Documentation
Details
| biocViews |
|
| Depends |
R
,
Biobase
,
RColorBrewer
,
xtable
,
lattice
,
methods
|
| Imports |
|
| Suggests |
|
| System Requirements |
|
| License |
LGPL-3 |
| URL |
|
| Depends On Me |
|
| Imports Me |
|
| Suggests Me |
|
Package Downloads