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bcSeq

This is the released version of bcSeq; for the devel version, see bcSeq.

All versions 3.24 (devel), 3.23 (release), 3.22, 3.21, 3.20, 3.19, 3.18, 3.17, 3.16, 3.15, 3.14, 3.13, 3.12, 3.11, 3.10, 3.9, 3.8, 3.7

Fast Sequence Mapping in High-Throughput shRNA and CRISPR Screens


Bioconductor version: Release (3.23)

This Rcpp-based package implements a highly efficient data structure and algorithm for performing alignment of short reads from CRISPR or shRNA screens to reference barcode library. Sequencing error are considered and matching qualities are evaluated based on Phred scores. A Bayes' classifier is employed to predict the originating barcode of a read. The package supports provision of user-defined probability models for evaluating matching qualities. The package also supports multi-threading.

Author: Jiaxing Lin [aut, cre], Jeremy Gresham [aut], Jichun Xie [aut], Kouros Owzar [aut], Tongrong Wang [ctb], So Young Kim [ctb], James Alvarez [ctb], Jeffrey S. Damrauer [ctb], Scott Floyd [ctb], Joshua Granek [ctb], Andrew Allen [ctb], Cliburn Chan [ctb]

Maintainer: Jiaxing Lin <jiaxing.lin at duke.edu>

Citation (from within R, enter citation("bcSeq")):

Jiaxing Lin, Jeremy Gresham, Jichun Xie, Kouros Owzar. bcSeq: Fast Sequence Mapping in High-Throughput shRNA and CRISPR Screens. doi:10.18129/B9.bioc.bcSeq, R package version 1.34.0, https://bioconductor.org/packages/bcSeq.

Generated from the package metadata; it may differ from the package's own citation.

Installation

To install this package, start R (version "4.6") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("bcSeq")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("bcSeq")
bcSeq PDF R Script
Reference ManualPDF
NEWSText

Details

biocViews ATACSeq, Alignment, CRISPR, ImmunoOncology, MultipleSequenceAlignment, SequenceMatching, Sequencing, Software
Version1.34.0
In Bioconductor sinceBioC 3.7 (R-3.5) (8.5 years)
License GPL (>= 2)
Depends R (>= 3.4.0)
Imports Rcpp (>= 0.12.12), Matrix, Biostrings
System Requirements
URLhttps://github.com/jl354/bcSeq
Bug Reportshttps://support.bioconductor.org
See More
Suggests knitr
Linking To Rcpp, Matrix
Enhances
Depends On Me
Imports Me
Suggests Me
Links To Me
Build Report Build Report, r-universe

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package bcSeq_1.34.0.tar.gz
Windows Binary (x86_64) bcSeq_1.33.0.zip (64-bit only)
macOS Binary (big-sur-x86_64) bcSeq_1.33.0.tgz
macOS Binary (sonoma-arm64) bcSeq_1.34.0.tgz
Source Repositorygit clone https://git.bioconductor.org/packages/bcSeq
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/bcSeq
Package Short Url https://bioconductor.org/packages/bcSeq/
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