TSSr
This is the development version of TSSr; to use it, please install the devel version of Bioconductor.
TSS sequencing data analysis
Bioconductor version: 3.24 · Package version: 0.99.21
TSSr package provides a comprehensive workflow on TSS data starts from identification of accurate TSS locations, clustering TSSs within small genomic regions corresponding to core promoters, and transcriptional activity quantifications, as well as specialized downstream analyses including core promoter shape, cluster annotation, gene differential expression, core promoter shift. TSSr can take multiple formats of files as input, such as Binary Sequence Alignment Map (BAM) files (single-ended or paired-ended), Browser Extension Data (bed) files, BigWig files, ctss files or tss tables. TSSr also generates various types of TSS or core promoter track files which can be visualized in the UCSC Genome Browser or Integrative Genomics Viewer (IGV). TSSr also exports downstream analyses result tables and plots. Multiple cores are supported on Linux or Mac platforms.
Author: Zhaolian Lu [aut, com] 
, Keenan Berry [aut, com], Zhenbin Hu [aut, ctb], Yu Zhan [aut, ctb], Tae-Hyuk (Ted) Ahn [aut, cph], Zhenguo Lin [aut, cre, cph] , National Science Foundation [fnd] (NSF 1951332)
Maintainer: Zhenguo Lin <zhenguo.lin at slu.edu>
Citation
From within R, enter citation("TSSr"):
Zhaolian Lu, Keenan Berry, Zhenbin Hu, Yu Zhan, Tae-Hyuk Ahn, Zhenguo Lin. TSSr: TSS sequencing data analysis. doi:10.18129/B9.bioc.TSSr, R package version 0.99.21, https://bioconductor.org/packages/TSSr.
Generated from the package metadata; it may differ from the package's own citation.
Installation
To install this package, start R (version "4.6") and enter:
if (!require("BiocManager", quietly = TRUE))
install.packages("BiocManager")
## The following initializes the development version of Bioconductor
BiocManager::install(version = "devel")
BiocManager::install("TSSr")For older versions of R, please refer to the appropriate Bioconductor release.
Details
| Version | 0.99.21 |
| License | MIT + file LICENSE |
| URL | https://github.com/Linlab-slu/TSSr |
| Bug Reports | https://github.com/Linlab-slu/TSSr/issues |
| Last updated | 2026-08-21 |
| In Bioconductor since | BioC 3.24 (R-4.6) |
| Downloads rank | 2447 of 2,456 |
| Source branch | devel |
| Build report | Bioconductor build system, r-universe |
| biocViews | Alignment, Annotation, Clustering, Coverage, DataImport, DataRepresentation, DifferentialExpression, GeneExpression, GeneRegulation, GenomeBrowsers, Normalization, PeakDetection, Preprocessing, Sequencing, Software, Transcription, Transcriptomics, Visualization |
| Package Short Url | https://bioconductor.org/packages/TSSr/ |
Documentation
To view documentation for the version of this package installed in your system, start R and enter:
browseVignettes("TSSr")| TSSr Vignette | HTML | R Script |
| Reference Manual | ||
| NEWS | Text |
Download
Follow the installation instructions to use this package in your R session.
| Source package | TSSr_0.99.21.tar.gz |
| macOS binary (arm64) | TSSr_0.99.21.tgz |
| macOS binary (x86_64) | TSSr_0.99.21.tgz |
| Source Repository | git clone https://git.bioconductor.org/packages/TSSr |
| Source Repository (Developer Access) | git clone git@git.bioconductor.org:packages/TSSr |
| Package Downloads Report | Download Stats |
Dependencies
Depends: R (>= 4.5.0)
Imports: BiocGenerics (>= 0.36.1), GenomeInfoDb (>= 1.26.7), GenomicFeatures (>= 1.42.3), GenomicRanges (>= 1.42.0), IRanges (>= 2.24.1), Rsamtools (>= 2.6.0), cigarillo (>= 0.99.2), data.table (>= 1.14.0), dplyr (>= 1.0.7), ggplot2 (>= 3.3.5), grDevices (>= 4.0.3), graphics (>= 4.0.3), methods (>= 4.0.3), parallel (>= 4.0.3), rtracklayer (>= 1.50.0), stats (>= 4.0.3), stringr (>= 1.4.0), txdbmaker (>= 1.0.0), utils (>= 4.0.3)
Suggests: BSgenome.Scerevisiae.UCSC.sacCer3, DESeq2 (>= 1.30.1), Gviz (>= 1.34.1), calibrate (>= 1.7.7), ggfortify (>= 0.4.12), knitr, pkgdown, rmarkdown, testthat (>= 3.0.0), withr