DOI: 10.18129/B9.bioc.rprimer    

Design Degenerate Oligos from a Multiple DNA Sequence Alignment

Bioconductor version: Release (3.16)

Functions, workflow, and a Shiny application for visualizing sequence conservation and designing degenerate primers, probes, and (RT)-(q/d)PCR assays from a multiple DNA sequence alignment. The results can be presented in data frame format and visualized as dashboard-like plots. For more information, please see the package vignette.

Author: Sofia Persson [aut, cre]

Maintainer: Sofia Persson <sofiapersson27 at gmail.com>

Citation (from within R, enter citation("rprimer")):


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biocViews Alignment, Coverage, MultipleSequenceAlignment, SequenceMatching, Software, ddPCR, qPCR
Version 1.2.0
In Bioconductor since BioC 3.15 (R-4.2) (0.5 years)
License GPL-3
Depends R (>= 4.1)
Imports Biostrings, bslib, DT, ggplot2, IRanges, mathjaxr, methods, patchwork, reshape2, S4Vectors, shiny, shinycssloaders, shinyFeedback
Suggests BiocStyle, covr, kableExtra, knitr, rmarkdown, styler, testthat (>= 3.0.0)
URL https://github.com/sofpn/rprimer
BugReports https://github.com/sofpn/rprimer/issues
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Source Package rprimer_1.2.0.tar.gz
Windows Binary rprimer_1.2.0.zip
macOS Binary (x86_64) rprimer_1.2.0.tgz
macOS Binary (arm64) rprimer_1.2.0.tgz
Source Repository git clone https://git.bioconductor.org/packages/rprimer
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/rprimer
Package Short Url https://bioconductor.org/packages/rprimer/
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