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This is the development version of ddCt; for the stable release version, see ddCt.

The ddCt Algorithm for the Analysis of Quantitative Real-Time PCR (qRT-PCR)

Bioconductor version: Development (3.3)

The Delta-Delta-Ct (ddCt) Algorithm is an approximation method to determine relative gene expression with quantitative real-time PCR (qRT-PCR) experiments. Compared to other approaches, it requires no standard curve for each primer-target pair, therefore reducing the working load and yet returning accurate enough results as long as the assumptions of the amplification efficiency hold. The ddCt package implements a pipeline to collect, analyse and visualize qRT-PCR results, for example those from TaqMan SDM software, mainly using the ddCt method. The pipeline can be either invoked by a script in command-line or through the API consisting of S4-Classes, methods and functions.

Author: Jitao David Zhang, Rudolf Biczok, and Markus Ruschhaupt

Maintainer: Jitao David Zhang <jitao_david.zhang at>

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PDF Analyse RT-PCR data with the end-to-end script in ddCt package
PDF How to apply the ddCt method
PDF Introduction to the ddCt method for qRT-PCR data analysis: background, algorithm and example
PDF   Reference Manual
Text   NEWS


biocViews DifferentialExpression, GeneExpression, MicrotitrePlateAssay, Software, qPCR
Version 1.27.0
In Bioconductor since BioC 2.5 (R-2.10) (6 years)
License LGPL-3
Depends R (>= 2.3.0), methods
Imports Biobase(>= 1.10.0), RColorBrewer (>= 0.1-3), xtable, lattice, BiocGenerics
Suggests RUnit
Depends On Me
Imports Me
Suggests Me
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